The Duke RBL, a BSL3 facility, contains a room specifically designed to use Hydrogen Peroxide Vapor to decontaminate laboratory equipment and has been operational for over a decade. This room currently utilizes a Bioquell Clarus™ C system with a 35% hydrogen peroxide solution and distribution system to disperse Hydrogen Peroxide Vapor into the room in a uniform fashion. In addition, we are evaluating the new Bioquell Z‐2 and Bioquell ProteQ systems that will provide increased capacity and flexibility to the reprocessing needs. A Battelle FDAfunded project, referenced previously, validated the decontamination of N95 respirators with Hydrogen Peroxide Vapor for over 50 cycles, with the reuse limiting factor being the elastic straps that started to show degradation. To address this, Duke plans to decontaminate and reuse N95s up to 30 cycles, with a Quality Assurance (QA) step to ensure both qualitative and quantitative degradation has not occurred. A cohort of respirators will be decontaminated with every cycle and will be used in standardized quantitative fit testing to ensure the integrity of the respirators is maintained over many decontamination cycles. During the tests performed by Duke, approximately 100 3M 1860 N95s, previously used in quantitative employee fit testing, were collected and hung from stainless steel wire racks in the Hydrogen Peroxide Vapor processing room. The Hydrogen Peroxide Vapor run consisted of the following five stages: Conditioning, Pre‐gassing, Gassing, Gassing Dwell and Aeration.

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• Bioquell Plc.

• Duke University

• Ecolab

• Coronavirus

• Hydrogen Peroxide

• Mask

• Decontamination

• Coronavirus

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